human prostate cancer cell line pc 3  (ATCC)

Journal: Pharmaceuticals

Article Title: Hydrophilic and Lipophilic Carbon Dots Impart Thermosensitivity to Doxorubicin Loaded Phospholipid Liposomes

doi: 10.3390/ph19050668

Figure Lengend Snippet: Comparative cytotoxicity of either empty or DOX-loaded PEGylated DPPC liposomes (Lip), or CD (Lip-CD) and CD-C16 (Lip-CD16) containing liposomes against PC3 cells following an 1 h incubation at ( A ) 37 or ( B ) 40°C. DOX concentration was 5 or 10 μM, while the concentration of empty liposomes corresponded to the liposomal concentration in experiments at the highest DOX concentration (10 μΜ). The cytotoxicity was assessed after a 24 h post-incubation period employing the standard MTT assay. Cell survival data are the mean of at least six independent values while error bars represent one standard deviation. The statistical significance was derived by Student’s t -test following the assignment: * p < 0.05, ** p < 0.01, *** p < 0.001; ns denotes no statistical significance, p > 0.05.

Article Snippet: The human prostate cancer PC3 cell line as well as the non-cancerous human embryonic kidney HEK293 of the cell bank from the Institute of Nanoscience and Nanotechnology, NCSR Demokritos, were purchased from the American Type Culture Collection (ATCC CRL-1435TM and CRL-1573TM, respectively; Manassas, VA, USA).

Techniques: Liposomes, Incubation, Concentration Assay, MTT Assay, Standard Deviation, Derivative Assay

Journal: Frontiers in Immunology

Article Title: PhIP-driven prostate cancer involves key molecular regulators and immune microenvironment modulation

doi: 10.3389/fimmu.2026.1782240

Figure Lengend Snippet: PhIP exposure induces cytotoxicity in RWPE-1 cells and downregulates SLC14A1 expression in PC-3 cells. (A) Immunohistochemical staining of SLC14A1 in prostate cancer tissues and normal prostate tissues based on data from the HPA database. (B) Cell viability of RWPE-1 cells after treatment with increasing concentrations of PhIP for 48 h, as determined by CCK-8 assay. (C) Protein expression levels of SLC14A1 in PC-3 cells following PhIP treatment, as assessed by Western blot. (D) Relative mRNA expression levels of SLC14A1 in PC-3 cells after PhIP treatment, as determined by qRT-PCR (**p < 0.01, ***p < 0.001).

Article Snippet: The human prostatic epithelial cell line RWPE-1 and the human prostate cancer cell line PC-3 were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Staining, CCK-8 Assay, Western Blot, Quantitative RT-PCR